RNA

Part:BBa_K1323001

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-07)

sgRNA (2): CRISPR Silencing of YFP

Single guide RNA (sgRNA) 2 is an RNA oligo that associates with dCas9 BBa_K1323002 and guides the CRISPRi complex to the coding strand of the YFP BBa_K1323010 at nt position 166 - 185. sgRNA is a fusion of crRNA (CRISPR RNA) and tracrRNA (trans-activation crRNA) - the 20 bp sequence is complementary to the target sequence and is linked to a hairpin loop that is similar to tracrRNA. When the sgRNA-dCas9 complex binds to the target sequence, it is expected to act as a programmable repressor and silence expression of the target gene.

This part was DNA synthesized by Bio Basic.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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